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Hi,
as far I can see your formula it is ok; anyway following you have step by step what you can do
first you calculate the
ΔCt= Ct (gene)- Ct (refference) where the refference it is the endojenous control, gene that you use for normalization in each sample.
the SD of this it is calculated by SD=(s1^2+s2^2)^0.5 where s1 the sd of gene and s2 the sd of reference gene
here you can normalize the expression if the gene1 in the A cells or tissu with the expression of the same gene in cell B or tissue B
by which the cellB or the tissue B it is your refference
ΔΔCt=ΔCt1-ΔCt2
the SD of ΔΔCt it is the same of the previous
then all the data can be shown as Log. with the formula 2^-ΔΔCt
