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Hi there!
I am preparing for my viva about " point mutations in the BCR-ABL kinase domain of chronic myeloid leukemia (CML) patients"
I need some help about:
A common point mutation found in CML is M244V....as we know that our body can't synthesize essential amino acids. Where M (methionine) and V (valine) both are essential amino acids...
The question is that how can we claim that the normal codon was coding for "methionine" and after a mutation it started coding for "valine"? although both are essential amino acids....hesis
Do we write "M244V" just to represent the nucleotide altered behind ...or an actual change in amino acid synthesis?
thinking!!
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Pcr product size determination for a fusion gene
26 August 2012 - 11:18 AM
Hi there!
I am facing a serious problem as I want to amplify a "Fusion oncogene" causing Chronic myeloid leukemia CML. I have got primer sequences from a research paper and it worked. The PCR is positive.
PROBLEM is that I don't know the exact size of my PCR product. Just roughly know that it is about 1.2 kb.
I tried NCBI blast . many hit and trials but ....
my Forward primer belongs to one gene i. e BCR gene on chromosome 22
and REVERSE primer belongs to the other gene i. e ABL on ch 9.
(These two genes combine to cause leukemia...fusion oncogene)
NCBI doesn't recognize both primers together, but blasts them separately to their respective genes.
Help please.
worried alot
I am facing a serious problem as I want to amplify a "Fusion oncogene" causing Chronic myeloid leukemia CML. I have got primer sequences from a research paper and it worked. The PCR is positive.
PROBLEM is that I don't know the exact size of my PCR product. Just roughly know that it is about 1.2 kb.
I tried NCBI blast . many hit and trials but ....
my Forward primer belongs to one gene i. e BCR gene on chromosome 22
and REVERSE primer belongs to the other gene i. e ABL on ch 9.
(These two genes combine to cause leukemia...fusion oncogene)
NCBI doesn't recognize both primers together, but blasts them separately to their respective genes.
Help please.
worried alot
