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de ifz

Member Since 08 May 2011
Offline Last Active Feb 14 2013 03:25 AM

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In Topic: how can we say that point mutations alter synthesis of essential amino acids?

04 November 2012 - 08:02 PM

Torf

Thankyou for the explanation about proteins. I appriciate that.

I am aware of this basic concept, its not a new thing to me.

To simplify my question please tell

"Do our body synthesize essential amino acids in a very less amount?"

rest I will interpret my confusion.

In Topic: how can we say that point mutations alter synthesis of essential amino acids?

04 November 2012 - 08:46 AM

but my concern is that when we say that our body cant produce essentil amino acids..then how we are talking about methionine alterations???

In Topic: Pcr product size determination for a fusion gene

30 August 2012 - 11:49 AM

bob1, on 28 August 2012 - 03:18 PM, said:

If you know which exons you are using and the size of the exons (try the BLAT tool at UCSC for getting the exons), can't you just add them up?

I know the exons...ok I try the adding up option...lets see if it works

In Topic: Pcr product size determination for a fusion gene

30 August 2012 - 11:45 AM

ascacioc, on 28 August 2012 - 11:41 AM, said:

Is it 780 bp with one primer? Or is it 780 with fwd and rev sequencing? The sequencing (as it is in the sequencing companies) is limited to 800-1000 bp. Moreover, don't sequence with the same primers you are doing the PCR, see here an explanation why:

http://www.protocol-...-ligation-help/

Better clone it in a plasmid and then sequence from the backbone of the plasmid towards the insert.

Andreea

Yes it is 780bp with forward and reverse seq. Lac of chemicals and limited budget of my project doesnt allow me the plasmid option

In Topic: Pcr product size determination for a fusion gene

28 August 2012 - 11:04 AM

thanx bob for the post...

the 8.5 kb size is actually encoding the whole cancer causing gene. suppose following is the fusion of BCR-ABL gene 8.5KB

123456789101234567
I am amplifying like

6789101234

for this I am unable to determine the size. I can roughly say that my product on gel appears to be 1.2 kb........??

The point mutations I am looking for belong to 1234 part. For which I got another set of primers. I got it sequenced and its size is 780 bp.

any further help plz.....
.

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In Topic: how can we say that point mutations alter synthesis of essential amino acids?

In Topic: how can we say that point mutations alter synthesis of essential amino acids?

In Topic: Pcr product size determination for a fusion gene

In Topic: Pcr product size determination for a fusion gene

In Topic: Pcr product size determination for a fusion gene

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de ifz

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In Topic: how can we say that point mutations alter synthesis of essential amino acids?

In Topic: how can we say that point mutations alter synthesis of essential amino acids?

In Topic: Pcr product size determination for a fusion gene

In Topic: Pcr product size determination for a fusion gene

In Topic: Pcr product size determination for a fusion gene

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