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Assuming the hemocytometer is like the one I use....
There should be a grid on the hemocytometer that you see through the microscope, with some bigger and smaller squares. Each square represents both a unit of area and a unit of liquid volume (because the specialized coverslip you put on it has a specific weight to it, in which only a specific amount of liquid can be under it). Based on the number cells per square, or set number of squares, you can calculate the number of cells you have in your sample, and from that calculate the volume needed to give you 5000 cells.
What I would do is find the brand of hemocytometer you have, go online and find the data sheet for it. That should tell you the units of area and volume that each square on the grid represent.
Does that help/make sense?
