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  2. → Viewing Profile: Topics: chipy

chipy

Member Since 05 Apr 2011
Offline Last Active Sep 04 2012 10:57 AM
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Topics I've Started

Dual Crosslinking using EGS

23 July 2012 - 10:33 AM

Hi there,

I'm trying to double crosslink a sample for TF chip.
I'm using EGS and I'm disolving it in DMSO (freshly prepared 1M stock).
I've being trying to use it at 5 mM in PBS, but as soon as I add it to my samples I see a precipitate.
Don't know how to get rid of it. Should I crosslink it also in DMSO??


THANKS!!

SDS precipitation after adding DNA binding buffer for column purification

04 July 2012 - 06:38 AM

Hi,

I'm using Zymo columns for purifying IP'ed DNA after elution with SDS and NaHCO3. SDS precipitates and I wonder if it could affect the yield of the purification.
I've tried heating the samples after adding the binding buffer and before adding the mixture to the coluns, but I wonder if Qiagen kits would be better or if there's another way to elute mi IP from Protein A Sepharose to avoid this issue.

Thanks.

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