change of planes! if I were to transfect cell lines with nacked- invitro transcribed- mrna, what would be the best and cheapest method? could I use DNA transfecting reagents? dose anyone have any experience with lipofectamin ltx( plus).
tnx pcr man. stabilizing RNA's backbon is an excellent approache and has been utilizied in a lot of rna studies, however naked not-inhanced rna has proven effective too. I couldn't find anything on the formulation though, other than " ringer solution" or "PBS" http://www.ncbi.nlm....pubmed/20961459
I was just wondering, how about using more than two enzymes in a single reaction, say, ApaI, XhoI and EcoRI , would it be any different, or as long as the glycerol's kept in range it would work just as well as sweet, old double digestion? how about fermentas's Fast Digestion buffer and enzymes? has any one tried a triple digestion with them?
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