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A common problem is using too much DNA in your PCR reaction. Try reducing the amounts used 10x or 100x.
#103612 Unpredictablity of PCR product
Posted
on 14 March 2011 - 06:26 PM
#103867 Placing dNTP in 64 C waterbath
Posted
on 16 March 2011 - 10:31 AM
I never did it, but don't think it will ruin it. The PCR reaction has higher temperatures...
Of course it depends on the time there, storing it at that temp will be harmful of course.
Of course it depends on the time there, storing it at that temp will be harmful of course.
#103600 Unpredictablity of PCR product
Posted
on 14 March 2011 - 01:44 PM
Hey, vortexing is not a good idea, because it breaks DNA, centrifugation shouldn't be a problem...
