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yes, you can use a microplate reader.
as long as all of the samples and standards are the same volume they will be valid.
but, you may alter the useful range of detection.
#121397 Microplate reader vs spectophotometer
Posted
on 12 October 2011 - 11:42 AM
#118974 Trizol and Protein extraction
Posted
on 05 September 2011 - 06:06 AM
Thanks for your reply, Raja. I just found out that ammonium acetate can also be used after phenol extraction.
Western blot is exactly what I planed to do in addition to RNA extraction. I will see the resuspension of the protein pellet.
Western blot is exactly what I planed to do in addition to RNA extraction. I will see the resuspension of the protein pellet.
