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Kamran

Member Since 08 Feb 2011
Offline Last Active Aug 03 2012 02:23 AM

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Group Active Members
Active Posts 41
Profile Views 1,282
Member Title Kamran
Age Age Unknown
Birthday December 10
Gender
Male

About me

My research interests
Cancer, Stem Cell Biology

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Posts I've Made

In Topic: Problem/qPCR, 2 peaks en melting curve

26 July 2012 - 09:07 PM

It may be due to residual genomic DNA in your RNA miniprep.

Try to digest your RNA with "RNase-free DNase" and then perform qPCR. Hopefully, you would not have that unwanted peak

In Topic: Pen/Strep from Sigma unit confusion

22 July 2012 - 09:56 PM

It's 100X concentrate;

Streptomycin 10'000 μg/ml

Penicillin 10'000 Units/ml

By adding 1ml of this antibiotics solution to 100ml (99+1) medium you would have 100μg and 100 Units of Streptomycin and Penicillin, respectively.

In Topic: Digest for insert orientation

22 July 2012 - 09:50 PM

you can also perform colony PCR using vector-insert primers to confirm correct orientation.

In Topic: Trouble Shooting for Transformation

13 July 2012 - 05:19 AM

the efficiency of competent cells can't be deduced from these controls.

You would have to do a control transformation in order to rule out this possibility.

In Topic: cell line cultured in suspension

06 July 2012 - 04:33 AM

primary hepatocytes ? or cell line?