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A PCR reaction requires 50 pMoles of primer (20 nucleotides in length) to be added to a 50 µl reaction. You have been supplied with an eppendorf (maximum capacity 1.5ml) which contains 66 µl of dried primer DNA. What do you to to end with 50 pMoles in your reaction?
Molecular weight of 1 nucleotide=660
How can I solve this ?
#99770 PCR reaction calculation
Posted
on 06 February 2011 - 12:05 PM
#99401 control DNA transformation- using pGEM kit
Posted
on 02 February 2011 - 12:16 PM
I am very new to this forum and cloning. I am working on a school project on cloning. I am only seeing white colonies on the control DNA plate, it means that my control worked because the control DNA is designed to produce white colonies. I also see white colonies with very faint blue centers on my plate with the inserted ligation. Can I assume that these colonies have my insert. Pls advise .
