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Grenouille

Member Since 01 Feb 2011
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Posts I've Made

In Topic: Canto II compensation beads - gfp

19 October 2012 - 05:56 AM

Thank you all. That's what I thought too. So I will continue to do my compensations with gfp positive cells what sometimes makes my experiments much more difficult (e.g. because of the low gfp intensities).
I also searched for something like gfp labeled beads (if something like this exists) but so far found nothing.

Again thank you all,

Grenouille

In Topic: experimental setting question (overexpression control)

28 June 2012 - 03:50 AM

thanks for your reply.
a protein with no (overexpressing)effects (like what?), or just any protein?

In Topic: what is mock cell line?

08 June 2012 - 03:30 AM

@dpo: I'm not completely sure if you are right with your example. An empty backbone transfection is an "empty backbone control" whereas your cells treated with the same transfection reagent (like Lipofectamine or s.th. else) like your whatever-plasmid-transfection but without any plasmid is your mock control. S.th. similar to WT, but stressed with chemicals/reagents in this case...

That's what I understood, but maybe I am wrong...

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