ask yourself why is it gone - is it just a mood, the team you work in, your boss, the environment......or is it that you expected something else?
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#125138 where's the passion now?
Posted
gebirgsziege
on 09 December 2011 - 04:34 AM
#125404 where's the passion now?
Posted
toejam
on 13 December 2011 - 04:25 PM
you should look at this:
#125497 where's the passion now?
Posted
lab rat
on 14 December 2011 - 10:18 PM
Frustration is weakness leaving the mind, like pain is weakness leaving the body. Persevere! The passion returns, if it was genuine.
I am just joking. When I get frustrated, I hug a pet and go for a long walk while listening to some sort of rock music with a heavy bass line. Viciously rearranging my environment helps, too.
I am just joking. When I get frustrated, I hug a pet and go for a long walk while listening to some sort of rock music with a heavy bass line. Viciously rearranging my environment helps, too.
#121084 pairwise alignment results explanation please
Posted
allynspear
on 07 October 2011 - 08:55 AM
After looking at your result, I think you have gotten as much out of it as possible. These genes don't appear to be that closely related by nucleotide sequence and there are far too many gaps and short stretches to give you any information. What you need is a protein alignment, but I tried looking for coding sequences within your sequences and I'm a bit confused. Each frame of both genes has at least a few stop codons in it, so are the sequences provided actually coding sequences? mRNA sequences? genomic sequences? It's important to know these things before you try to interpret any alignment.
As far as similarity and identity goes, for nucleotide alignment, there is no difference unless your sequence has ambiguous characters like Y, H, W, N, etc. For protein alignments, identity means exactly the same amino acid in a position, but similarity can mean amino acids with similar properties:
Serine and Threonine: Both hydrophillic, hydroxyl
Isoleucine and Leucine: Both hydrophobic, aliphatic
Aspartic acid and Glutamic acid: Both hydrophillic, acidic
When you get a protein alignment, repost and people can take a look.
Best of Luck.
As far as similarity and identity goes, for nucleotide alignment, there is no difference unless your sequence has ambiguous characters like Y, H, W, N, etc. For protein alignments, identity means exactly the same amino acid in a position, but similarity can mean amino acids with similar properties:
Serine and Threonine: Both hydrophillic, hydroxyl
Isoleucine and Leucine: Both hydrophobic, aliphatic
Aspartic acid and Glutamic acid: Both hydrophillic, acidic
When you get a protein alignment, repost and people can take a look.
Best of Luck.
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