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  2. → Viewing Profile: Topics: Shrubal

Shrubal

Member Since 02 Jan 2011
Offline Last Active Dec 06 2012 09:03 AM
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Topics I've Started

role of water in PCR

06 December 2012 - 09:00 AM

Hello!

I was wondering if anyone could explain the specific role of water in a PCR reaction. I was wondering if the addition of water could be completely eliminated and instead compensated by adding more template if I had a very low concentration of template.

Thanks!

minimum amount of RNA for RT_PCR

06 December 2012 - 08:50 AM

Hey all!

I have a very low concentration of RNA from my samples (1-2ng/uL i.e. a total of 50-100ng). I usually use 500ng of total RNA for my reverse transcription-PCR (reverse transcription) and was wondering if anyone has had any experience (and therefore suggestions) with using a low quantity of total RNA.

Thanks!

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