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  2. → Viewing Profile: Posts: Paulgs3

Paulgs3

Member Since 22 Nov 2010
Offline Last Active Jun 13 2013 02:10 AM
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Posts I've Made

In Topic: CFU measurments

27 April 2013 - 03:37 AM

If it was me, I'd gather more than two data points.

In Topic: CFU measurments

26 April 2013 - 04:26 AM

I agree there could be a ton of reasons why: viability caused by incubation time, media formulation, incubation temp, shaker speed....

A log difference in cfu is rather significant to me.

In Topic: odd crystal structure in my matrigel

20 July 2012 - 07:45 AM

I've seen those in urinalysis specimens. I'm not familiar with what you are doing, but are you using antibiotics?

In Topic: Not sure how to make HEPES or MOPS buffered media

13 July 2012 - 06:54 AM

I'm lazy I just buy it as a solution and dilute as necessary.

In Topic: Resuspending bacteria to a certain OD600

29 June 2012 - 02:13 AM

You're on the right track.  The formula you want is the old: C1V1=C2V2.

Edit for clarity:

(0.5)(2ml) = (1.7)(x)

X= 0.58ml

Take 0.58 ml of the 1.7 OD and QS to 2ml

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