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Posted
schaapje_86, on 23 January 2013 - 06:03 AM, said:
Maybe this is a stupid question, but I am going to ask it anyway:
I would like to cut out an insert from my plasmid (pET28c) between restriction sites Nhe1 and Not1 and ligate the plasmid back to it's orginial state without the insert (no I don't have the original plasmid anymore). However, obviously these restriction sites don't generate compatible sticky ends. Does anyone know how to recreate this plasmid?
Many thanks!
I would like to cut out an insert from my plasmid (pET28c) between restriction sites Nhe1 and Not1 and ligate the plasmid back to it's orginial state without the insert (no I don't have the original plasmid anymore). However, obviously these restriction sites don't generate compatible sticky ends. Does anyone know how to recreate this plasmid?
Many thanks!
Klenow polymerase
Making blunt ends.
Do a google search, you should be able to find helpfull information.
If something is not clear, just ask, but I think if you look those two things up, you will know what to do!
