I would like to cut out an insert from my plasmid (pET28c) between restriction sites Nhe1 and Not1 and ligate the plasmid back to it's orginial state without the insert (no I don't have the original plasmid anymore). However, obviously these restriction sites don't generate compatible sticky ends. Does anyone know how to recreate this plasmid?
Making blunt ends.
Do a google search, you should be able to find helpfull information.
If something is not clear, just ask, but I think if you look those two things up, you will know what to do!