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  2. → Viewing Profile: Posts: tihong10

tihong10

Member Since 17 Nov 2010
Offline Last Active Apr 14 2013 11:37 PM
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Posts I've Made

In Topic: Continuous growth of E. coli on AMP LB plate

25 February 2013 - 06:43 PM

Yes, my plates were spreads of liquid aliquots...I will try your dilution suggestion...Thank you!

In Topic: Help clarify mucoid and non-mucoid Pseudomonas aeruginosa

05 February 2013 - 09:47 AM

Thanks for the interesting looking article!

In Topic: Freeze transformation mixture?

31 January 2013 - 02:20 AM

I see, thanks for the tip.

I tried plating the frozen transformation mixture and nothing grew : /

In Topic: The Issue of Freezing and Using colonies of Tranformed DH5a

31 January 2013 - 02:15 AM

I am remaking my plates and using a new source of ampicillin and a 2hr water bath - hopefully the results come out better than the first time.

Thanks bob1 and phage434!

In Topic: Why tranform using DH5 alpha -> SM10 λpir??

31 January 2013 - 02:12 AM

Sorry for the delayed response:

I am using the vector pdsRed-express2; I am not sure about the origin of replication though...

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