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ghelrefay → phage434
Dear iwant know why dot blot obtained with samples please reply me i diagnosis TB by PCR Dot Blot
Oct 31 2010 10:19 AM
ghelrefay → Alsai
please iwant know what the amount of biotinylated probe added to hyberdization solution 5ml to obtain the concentration of prob 200ng with know that the prob is pcr product of biotinylated primer i made diagnosis of TB by pcr dot blot not result obtain for all pulmonary TB sample but give result with positive control h want know solve for my problem
Oct 29 2010 04:15 AM
ghelrefay → HomeBrew
please iwant know what the amount of biotinylated probe added to hyberdization solution 5ml to obtain the concentration of prob 200ng with know that the prob is pcr product of biotinylated primer i made diagnosis of TB by pcr dot blot not result obtain for all pulmonary TB sample but give result with positive control h want know solve for my problem
Oct 29 2010 04:14 AM
ghelrefay → phage434
please iwant know what the amount of biotinylated probe added to hyberdization solution 5ml to obtain the concentration of prob 200ng with know that the prob is pcr product of biotinylated primer i made diagnosis of TB by pcr dot blot not result obtain for all pulmonary TB sample but give result with positive control h want know solve for my problem
Oct 29 2010 04:07 AM
ghelrefay → HomeBrew
how ican solve my problem in dot blot for pcr product which no result for sample on dot blot but give result on agarose in second amplified while positive control give result on dot blot
Oct 25 2010 12:18 PM
ghelrefay → phage434
hi you can help me in my prblem in dot blot for pcr product which not obtain result for al samples except the positive control give result i diagnosis tb
Oct 25 2010 11:43 AM
