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chandra3316

Member Since 20 Sep 2010
Offline Last Active May 02 2013 02:57 AM

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In Topic: HeLa growing cloudy

12 March 2013 - 04:18 AM

I am sure it is not contamination.. Mostly because of maintaining conditions...I guess I Used lot of spirit to clean my hands before placing it in the incubator. so it caused the stress to them..

what do you say??
please discuss possibility for this or high medium or thawing(DMSO)....)

In Topic: marker of inflammation

11 March 2013 - 09:40 AM

NO is also needed for angiogenisis, and survival,immunity, other physiological functions depending on the organ they produced. You better concentrate on interleukins (IL6 etc..)

In Topic: marker of inflammation

11 March 2013 - 12:13 AM

not like that...

In Topic: please help with my western blots!

01 March 2013 - 09:46 PM

Lyse the cells in the liquid nitrogen.. that will avoid jelly....

:)

In Topic: Bile test for Lactobacillus

09 August 2012 - 05:41 AM

Hi!
friend I think the same,of Veteran's answer. Since bile salts contain steroid nucleus, they gets destroyed upon autoclaving. like plant hormones we use. (your doubt about interference is reasonable) So you better add them after the Autoclaving. with in the laminar air flow(use flame if possible to maintain strict sterile condition during addition) to avoid precipitation.

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chandra3316

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