Recently, I read some papares about the Streptolysin O (SLO) Antigen which I want to form a pore at cell membrane. And what attracted me was the method of stabilized this protein. Many companies used this method, just like http://www.aaltobioreagents.ie/datasheet/AW6093-b%20da.pdf ,but I cant find any information about it. And I analysed the sample (aalto bio inc.) with SDS-PAGE. And I was very surprised, because the protein was in souble aggregation. Describled as the vendor, the SLO can be more stable in this form. I want to know the methed? Is there some one know ? The SDS-PAGE aatached below.
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