Ameya P

read, read, read, read, read, read, read, read, read, read, read, read, read, read,read, read, read, read, read, read, read,read, read, read, read, read, read, read,read, read, read, read, read, read, read,read, read, read, read, read, read, read,read, read, read, read, read, read, read,read, read, read, read, read, read, read,read, read, read, read,
plan,
write


Regards,
p

Writing proposals, outlines for grants and projects is a tiresome duty if you want to stay in research and have no permanent position or need additional money for a project....
as an alternative way to get money crowd funding becomes more and more important, and next to websites where this is offered for all types of projects, also sites that are only for funding science projects are created.
Here is a short paper about this in Nature Neuroscience.

A few examples:

Kickstarter is a website for all kinds of projects and ideas that need money (arts projects, start up companies, etc)

Fundscience (non-profit, USA-based, only for science projects)

MyProjects (UK, only for cancer research projects)

Open Genius (non-profit, science only, still in development I think)

Because of the not that high amounts of money you can get, it's perhaps a good idea for pilot projects, feasibility studies or funding gaps. Have a look.

sounds familiar? ....

I mangled this Kesha's song  during a long road trip........my apologies to Madame Curie as well....




Tick Tock

Wake up in the morning feeling like Marie Curie
Put nerd glasses on, I’m out the door,
I’m gonna hit labrat city
Before I leave, brush my teeth with a bottle of Jack
Cause when I leave for the lab, I ain’t coming back

I’m talking ‘bout – protein to extract, xtract
Ugly boss on my back, back
Corny lab jokes to crack, crack
And lots of buffer powders to mix, mix
Cells on the slide to fix, fix
Lab meeting from four till six, six

[CHORUS]

Can’t stop, must hurry up
Still need to do PCR crap
Tonight, I'm gonna write
‘til I see the sunlight
Tick tock, on the clock
draat…my PAGE gels always suck, no
Woah-oh oh oh
Woah-oh oh oh

hianghao, on 07 March 2011 - 09:42 PM, said:

It has to do with salt and pH. For the DNA to bind to silica you need lots of salt and a low pH (below 7.5). To release the DNA you elute with a solution that is poor in salt and has a pH >7.5 (the EB in the Qiagen kit is at pH 8.0 I think).

for those who can't tell sizes apart (uhmmm...I mean, can't compare, estimate and calculate like moi... ) check THIS out.. btw..you've to move the button along the bar for your total viewing pleasure (took me 5 min to figure this out......)

I need the answers of end-of chapter questions of molecular biology of the cell from alberts. Is there anyone having pdf file of it?
thanks in advance..

Kira, on 09 January 2011 - 04:05 PM, said:

Not a typo.  You laid it out in your first sentence.  A nucleoside is simply the sugar + base, so ATP is a nucleoside (sugar + adenosine base) tri-phosphate (3 phosphates).  If you were to say nucleotide triphosphate, you'd be redudantly saying it had phosphates, since the term nucleotide implies a phosphate...then you go and say it contains phosphate a second time.


This is similar to how people say "ATM Machine", it seems to sound fine, but it is certainly not the correct thing to say. ATM stands for Automated Teller Machine...so when you say "ATM Maching", you're saying "Automated Teller Machine Machine".  This is pretty equlivant to saying nucleotide triphosphate instead of nucleoside triphosphate.

The Ostrich
A man walks into a restaurant with a full-grown ostrich behind him.
The waitress asks them for their orders.
The man says, "A hamburger, fries and a coke," and turns to
the ostrich, "What's yours?"
"I'll have the same," says the ostrich.


A short time later the waitress returns with the order.  "That will
be $9.40 please."  The man reaches into his pocket and
pulls out the exact change for payment.
The next day, the man and the ostrich come again and the man
says, "A hamburger, fries and a coke."




The ostrich says, "I'll have the same."


Again the man reaches into his pocket and pays with exact change.


This becomes routine until the two enter again. "The usual?"
asks the waitress.
"No, this is Friday night, so I will have a steak, baked potato and
a salad," says the man.


"Same," says the ostrich.
Shortly the waitress brings the order and says, "That will be $32.62."


Once again the man pulls the exact change out of his pocket and
places it on the table.


The waitress cannot hold back her curiosity any longer.  "Excuse me,
sir.  How do you manage to always come up with the exact change
in your pocket every time?"


"Well," says  the man, "several years ago I was cleaning the attic and
found an old lamp.  When I rubbed it, a Genie appeared and offered
me two wishes..   My first wish was that if I ever had to pay for anything,
I would just put my hand in my pocket and the right amount of money
would always be there."


"That's  brilliant!" says the waitress.  "Most  people would ask for a
million dollars or something, but you'll  always be as rich as you want
for as long as you live!"


"That's right..  Whether it's a gallon of milk or a Rolls Royce, the exact
money is always there," says the man.


The waitress asks, "What's with the ostrich?"


The man sighs, pauses and answers, "My second wish was for a tall chick
with a big butt and long legs who agrees with everything I say.."

here's a review with most of the techniques:

S. Calin Andras, J. Brian Power, Edward C. Cocking and Michael R. Davey (2001): Strategies for signal amplification in nucleic acid detection. Molecular Biotechnology 19(1): 29-44.

printing double sided. makes me feel "environmentally friendly"

Genome by Matt Ridley
it is a 23 chaptered SMALL book in which each chapter tells a story
about one gene discovered on that chromosome...

nice one

Hi

There is a new electrophoresis buffer out called SB, which is just NaOH (10mM) pH adjusted to 8.5 with boric acid (works out at about 2.6g/L).   Note the lack of Tris, which is the expensive part of TBE and TAE, but works just the same, in fact you can electrophorese at much higher voltage, I routinely run gels at 200v for 0.5 hr with the same resolution and separation as 2 hours at 50v in TBE.

Check out BioTechniques 36:2 pp214-215 2004 for the article.

HomeBrew, on 07 August 2010 - 06:43 AM, said:

To summarize, we disagree on two point.

1-The degree that humanity plays in the this global warming trend.

2-How humanity should allocate our limited and finite resources.

Why are those two points of contention important? I have come to realize it is because they overlay a more basic question, "Do we have sufficient time to adapt our civilization or do we need to buy time."

If we have sufficient time to adapt our civilization, I agree, we should spend our finite resources adapting. If humanity does not have enough time, it would be logical to expend some resources buying time, so we can complete the adaption process.

I stand on the side of "We don't have enough time". People take a lot of time to get their act together.  Humanity has to get through the recognition stage, the denial stage (I think we are here), the anger stage, the acceptance stage, the long planing stage and finally the implementation stage.

Knowing people's propensity to procrastinate and dash madly to meet dead lines, the same would probably happen during this challenge. And worryingly, we will only know when the deadline has arrived when our crops fail, our seaports flood and shortages of goods due to disruptions from single point failures in the global supply chain. And yes, I recognize not all system failures will hit at the same time. But I believe those short few years will not provide enough time to adapt. Nor would the warning signs be recognized as such.


I also stand on the side that "Humanity do make a significant impact to the global warming trend."

Thus humanity can "buy" time to adapt by moving to green technology which has reduced emission of carbon dioxide. However I was thinking a step more than that. I was thinking of actual expenditure of resource/energy to remove carbon dioxide from the atmosphere (my preference is to carbonize wood and storage of the charcoal produced underground). And yes, the dreadful solar shield idea with sulfur dioxide in the upper atmosphere would come under the idea of "buying time". Dreadful because knowing human behaviour, once the solar shield is up, people will stop bothering and make no attempt to adapt civilization. And when the shield goes down, we will get hit with all the changes in an instant, without even the few decades to adapt that we would receive with this warming trend.