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Yes I'm using black plate (the bottom is black as well) and I paid attention to bubbles as well.
So for the gain, my method is not correct?
You are saying that is better to assume the positive control (protease+substrate without inhibitor) as the well will produce the highest fluorescence instead of to insert a number, right?
The filters are long pass.
Thanks I will have a look to the videos!
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In Topic: FRET based inhibition assay
11 March 2013 - 12:25 PM
Hi,
I would like to know if there is something wrong in my protocol and how I can improve my measurements in term of signal noise and reproducibility. I was wondering if it's better to use the bottom optic instead of the top one and if the method I used to adjust the gain is correct. Can you suggest me any tips to improve my measurements?
Thanks
I would like to know if there is something wrong in my protocol and how I can improve my measurements in term of signal noise and reproducibility. I was wondering if it's better to use the bottom optic instead of the top one and if the method I used to adjust the gain is correct. Can you suggest me any tips to improve my measurements?
Thanks
