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  2. → Viewing Profile: Posts: Gradstudent78

Gradstudent78

Member Since 22 Jul 2010
Offline Last Active Dec 02 2012 05:04 PM
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Posts I've Made

In Topic: Lab refrigerator

24 October 2012 - 08:16 AM

Not worried about it being explosion proof, I'm not putting anything in there where that is a risk (only Elisa kits).  Only need ~4C, definitely planning on a refrigerator only unit, I have more then enough freezer space already.

In Topic: Making a Phosphate Buffered Saline

23 October 2012 - 06:34 PM

Great thanks.   I'd still be curious if my calculations were right if anyone wants to comment on them.

In Topic: Making a Phosphate Buffered Saline

21 October 2012 - 12:01 AM

Ok this is what I've come up with for the amounts (making 1 Liter instead of 500 mL, a 10x solution would probably just be a waste as I only have to use this every couple of months):
I used Henderson-Hasselbalch equation for the sodium phosphate concentrations, using a pKa value of 6.865 (I'm a little confused on this as some places say it's 6.86 and some say it's 7.21)

7.2 = 6.865 + log (Disodium phosphate Dodecahydrate/Monosodium phosphate monohydrate)

Results:
24.490 grams of Disodium phosphate Dodecahydrate
4.363 grams of Monosodium phosphate monohydrate

For the NaCl I calculated:
8.766 grams of NaCl

In Topic: ELISA Incubator

25 September 2012 - 02:44 PM

Thanks, I finally picked one up.  It's a labnet incubator (which was pretty inexpensive), I'm testing it this week and I'll keep an eye out for hot spots and any edge effects in my assays.

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