I recommend you read a couple of technique papers on pancreas isolation. Due to the amount of RNAses present in the pancreas, isolating intact RNA from the pancreas is extremely difficult. In our lab, we do a quick resection (under 10 seconds) and snap freeze the tissue. We then pulvorize the tissue while frozen and hit with TRIZOL immediately.
There are other techinques that you can find on the web. You will have to find what works best for your lab setting. Just make sure to do Agilent for your RNA and calculate RIN to make sure you don't have degraded RNA.
Doggie ScienceMember Since 13 Jul 2010
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14 Jul 2010 - 03:35