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TTT

Member Since 26 May 2010
Offline Last Active Nov 19 2010 03:12 AM
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cartoonyangTTT

I can not send you message because i am a new one, so i just write here. I hope you can help me! I am done today, and i need your suggestions and continue...
May 24 2011 07:48 AM
cartoonyang's Photo

cartoonyangTTT

i am going to use Propidium iodide(sigma4170), Hoescht and YO-PRO-1 (Y3603)dyes to stain myoblast cells for apoptosis assay. I saw you post this question last year. I guess you have already done . So could you tell me the protocol of staining? I use 1mixstaining solution 1uM YO-PRO+10ug/mlPI+Hoechest(33342(1:500) ) to incubate myoblasts 30min in 37degree incubator, and then wash with PBS, and t...
May 24 2011 07:47 AM
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