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Hola I think it´s be better inoculate your sample without imidazole, as far as I know you have to concentrate your positive fractions in order to inject an smal volume to the animals so, by the way if you concentrate by ultrafiltration dialyze in the ultrafiltration device your sample. For instance you have 15ml of sample and need concentrate to 2 ml for inject, so ultrafiltrate to the less volume possible and dilute again with your buffer at 3 volumes so making twice this you have at the end an imidazole conc or 25-30mM less toxic.or dialyze in a high volume in dyalisys bag and concentrate afterwards.lethal dose of imidazole in rat about 1g/k. Buena suerte
#123967 getting rid of imidazole in purified protein?
Posted
on 20 November 2011 - 11:49 PM
