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muntasir

Member Since 21 Apr 2010
Offline Last Active Nov 21 2012 06:15 PM

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Topics I've Started

Sequence submission in GenBank using Sequin

14 August 2012 - 08:50 PM

For the first time I am going to submitt some viral cRNA sequence in GenBank. I have prepared the sequence data using Sequin. Now I need to email them to GenBank staffs. I query is, "What information I must provide with the email?" and do I need to add any detail about my study?

Please share your experience.

qPCR standard curve slope (m)

31 May 2012 - 07:42 AM

I am using quantitive Real Time PCR for detection of etiologic agents from patients samples. Our current target is 33 pathogens. One company has provided us plasmids to be used as positive control. For each target we need to run standard curve per quater month and use the slope (m) and Y-intercept © for quantification of our samples.

For each target we are getting different slope (m). My question is "what is the ideal range for slope of qPCR standard curve?"