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try ems mutagenesis/activation tagging/x-ray mutagenesis rotocol in Arabidopsis protocol book by - Glazebrook and Weigel. Will give you some starting material. A population of around 100000 seeds that can be done in 2-3 50 ml tubes for arabidopsis (?) may b be good to start but then you have to design the screen based on what you are looking for.
Hope that helps
