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jasmina

Member Since 16 Feb 2010
Offline Last Active Today, 08:53 AM

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italy

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Hello,
I have a problem with western blot, I'm trying to purify one membrane protein expressed in the brain the MW is around 120 KDa. I'm using in vitro model of 293 cells that express this protein. After many steps of solublisation in RIPA buffer, max amplitud of sonication, max centrifugation, quantification of proteins, I boil the samples for 5min, and load in the gel. I'm unable to see this protein by western !!! if you have any idea, please help, thanks

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#150480 Interview for postdoc

Posted Curtis on 16 February 2013 - 08:06 PM

just be confident. don't panic.....and then when you get the job, find one for me too Posted Image

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