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ascacioc, on 10 October 2012 - 08:58 AM, said:
It is a problem of the running buffer; most probably it contains the double amount of glycine. Make a fresh one.
I usually dilute a 10x stock of running buffer which contains 140g Glycine, 30g Tris-Base, 0.5g SDS per 1 litre. Never had a problem before with the running buffer.
