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badguy

Member Since 03 Feb 2010
Offline Last Active Jun 17 2013 12:20 AM
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Topics I've Started

DNA polymerase for GC rich template?

08 January 2013 - 11:55 PM

In among all the brands of DNA polymerase, which one stand out among all?
I found out Bioline's MyTaq look pretty good when compare to other vendors' DNA polymerase with DNA template of 61%GC.
Anyone uses Bioline's product before?

Thermal cycler recommendation

17 December 2012 - 09:30 PM

Any good thermal cycler to recommended?
currently i laid my eye on Biorad's C-1000. any other better than this?

Lysis method for single human embryonic cell

23 September 2012 - 11:33 PM

I need help from you guys regarding lysis method for single human embryonic cell.

Other than using Proteinase K and mild detergent (eg. tween-20), is it posible to use boiling lysis method or heat denaturation just like in colony PCR?
I will use this sample to do PCR. Thanks in advancePosted Image

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