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biolcrazy

Member Since 16 Jan 2010
Offline Last Active Feb 13 2013 02:19 PM
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Posts I've Made

In Topic: IPTG induction doesn't work:(

21 August 2012 - 07:13 AM

1. The cells should be at an OD600 of at least 0.6-0.8 when you are adding IPTG---that would be log phase of growth
2. Do you add freshly prepared IPTG? Maybe your IPTG stock is old.
3. Induction time (and temperature) greatly varies from one protein to another. Try at least 4-8 hours at 30C ...take an aliquot every hour of induction. Alternatively you could try O/N induction at 16C

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