Find content
Not Telling
After making serial dilution and the solution will be plated out.
For example, I prepare dilution like this.
10^-1
10^-2
10^-3
10^-4
10^-5
Change pipette tip when transferring mixtures to the next dilution in each step.
However, I'd like to know that Can I use the same pipette tip when plating out from high to low dilutions or not (from 10^-5 to 10^-1)? The number of bacteria will be the real number or not?
Thanks for answer.
