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The biggest problem is likely to be degradation of the labile compounds in the medium such as l-glutamine. However, remember that you incubate cells in this medium for several days at 37 without problems, so I don't think leaving it in the waterbath overnight will be too much of a problem so long as you don't make a habit of it.
#91953 cell culture media
Posted
on 10 November 2010 - 04:06 PM
