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No, T4 ligase is sufficient for blunt end ligation. The provided ATP will provide the phosphate linkage.
#151554 ligating blunt ends of a linearized plasmid, is kinase necessary?
Posted
on 04 March 2013 - 02:15 PM
#136451 What does "Under Editorial Consideration" mean?
Posted
on 26 June 2012 - 11:39 PM
before being sent to te reviewers an editor usually has a quick look at your paper - to see if you meet all the criteria of the journal (e.g. quality, format, scientific novelty, language etc). this is editorial consideration.
the second sentence means that you have passed this initial check and that your ms has (or will be) sent to the reviewers.
the second sentence means that you have passed this initial check and that your ms has (or will be) sent to the reviewers.
#134089 Any free software for drawing genes?
Posted
on 06 May 2012 - 01:19 PM
Hi
I have collected all softwares that others have written here in my wordpress site:
https://babakmemari....lysis-software/
-----
Babak
I have collected all softwares that others have written here in my wordpress site:
https://babakmemari....lysis-software/
-----
Babak
#123315 Ligation needed after SDM with PfuX?
Posted
on 09 November 2011 - 12:13 AM
If you use complementary primers your product should be circular. If you are not quite sure than draw a model of the PCR with plasmid, direction of primers and you will see how your product will look like.
#123934 Ligation needed after SDM with PfuX?
Posted
on 20 November 2011 - 08:20 AM
I have done such a similar SDM as you did. In fact, it's not needed to ligate the PCR products. After PCR, the DNA products are circular but with two nicks. The nicks will be ligated when they are transformed into E. coli.. But, Dpn I should be used before transformation. Or else, your SDM success rate will be very low.
