I would like to thank everybody for expressing his/her sympathy to my case. Special thanks for the practical clues too.
Well, teaching sounds great but in my country for 4th-5th year the only one University that provides study of Natural sciences has plenty of unoccupied positions for students - nobody wants to study life sciences, chemistry or physics because they are difficult and have no realisation here. Plus, all teaching positions are occupied until the rest of the eternity.
Research.....ah, the life here is as expensive as in the rest of EU (for some things like energy we pay much more) but salaries....I would be lucky if I get 250 Eu per month. And the system for grants is still very corrupted. And the whole society here is against people like me - here only the practical jobs are respected. This is why I wrote my topic....and I think I got some clues :-)
Finally, I am 35 years old and it is absolutely neccessary to give a birth as soon as possible.
In the name of God, I have been working all my life for this!
You see - it is not a matter of psychological depressia but of a real problem......that I hope to resolve somehow :-)
One more time thank all of you :-)
Is it possibly to compare gene expression of different genes within a single sample using semi-quantitative RT-PCR? For example, if I ran an identical number of cycles for the same DNA sample with primers for three different genes, and then ran the PCR product on an ethidium bromide gel, would this reflect their relative gene expressions (aka, if one band for one gene were brighter than another band for another gene?) Or is it impossible to make this deduction?
I'm sorry if this is a bad question, I was just curious how exactly this would work!
I am not competent at all, but basically I have the same task - to compare gene expressions.
Why don`t you have a look on these two papers, section Material and Methods?
Boonmars T, Wu Z, Nagano I, Takahashi Y. 2004 Parasitology, 128, 323-332
Wu Z, Nagano T, Boonmars T, Takahashi Y. 2005 Parasitology, 131, 373-381