Nephrite

Hi, Goggi.......
I was looking around (sneaking for advices ), I saw your post and decided to write you....
How are you doing 6 months later?
I suppose your are less than 30, I see that your English is quite good and......you are in Great Germany.
If you don`t like science and feel disappointed, why don`t you start looking for a job in the farmaceutical industry? Like Clinical trial assistant or junior Clinical research associate? They don`t need PhD holders but young and energetic people.
If I only knew this few years ago.....I would not pass through the Hell named PhD.
However, I wouldn`t have met my future husband, too

Thank you!

I wanted to send you some of my melting curves;
1. one positive sample with a single peak;
2. one NTC (which has no peaks);
3. one with the specific product and primer-dimers (which are smaller as Trof says and are located on the left side);
4. one with two products (two peaks, both located on the right side)
but I am not allowed to upload emf files.
Here I have a veeeeeeeery old PC and even don`t know with what kind of program to open them.
Never mind.
I don`t know what we should do if we have variant 3 in our results (maybe just optimization) but I know that variant 4 is the last thing that we want to see when we do qPCR :-)))))))))))
I think, this should mean either contamination (because primers are not very specific) or two specific products which result from splicing variants. This is what I`ve been told.

When I started to introduce myself with the real-time Trof gave me the Best Advise I Have Ever Got, which was ''Just start doing your real-time experiments and see what will happen. No reason to worry about the problem before you have it.''. So simple and effective.

I hope this will help also you. We can not become experienced if we do not gain experience :-)

Good luck :-)

Thank you!

Thank you for the support. I am Bulgarian, by the way, so.......you have a really good idea how it is. I sincerely hope to find a position as an CRA without an experience (I already made some steps) or at least to win a position in the Medical Academy - the Klinical lab or Molecular genetics Departments. Bulgaria is a so small country.....