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biocrazy

Member Since 30 Oct 2009
Offline Last Active Aug 23 2012 05:45 PM

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In Topic: Problem in Vector-Insert digestion result

07 June 2012 - 06:49 PM

Are you sure there is no other restriction site in the vector for the enzymes that you used?

If yes, it may undergone a non-specific digestion at some other place in the vector. You can try again by reducing the incubation time.

Good luck!!