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skad

Member Since 11 Oct 2009
Offline Last Active Mar 21 2012 09:33 AM
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#126258 Mechanism of transgene integration (from expression vector to the host genome)

Posted skad on 02 January 2012 - 12:53 AM

Hi All,


May I know if you have any idea/comment/reference answering the following question:

How does a transgene (in vector) integrate to the host genome? (e.g. in glass bead method, neither biolistic nor agrobacterium).

I already cut some parts (NdeI-PciI) of the vector (pUC18) and wondering if it affects the integration procedure. Is there any recombination-like procedure same as gateway?

  

Thanks in advance...pUC18.jpg


#13779 Important literature for real time PCR users

Posted tea-test on 29 January 2009 - 09:58 AM

here's the next bunch :lol:


#13777 Important literature for real time PCR users

Posted tea-test on 29 January 2009 - 09:50 AM

View Posttea-test, on Jan 29 2009, 07:46 PM, said:

Hello,

I have gathered some important documents with many useful information regarding real time PCR.

here are the next ones


#13775 Important literature for real time PCR users

Posted tea-test on 29 January 2009 - 09:46 AM

Hello,

I have gathered some important documents with many useful information regarding real time PCR.


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