I use both protocols (and normally I dont check amount of protein, as I use the same amount of cells and I am not working with cell death or apoptosis or something that kill my cells).
If I want to see some specific protein modification (as sumoylation) I use laemli directly on the samples, but to avoid the viscosity that is how I do it:
Recolect the cells
Add laemli buffer 1x 120ul
Boil for 2 minutes
Boil 5 minutes
I have no problem with this easy protocol
laurequilloMember Since 24 Sep 2009
Offline Last Active Apr 16 2013 11:25 PM
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- Member Title The Goddamn Batman!
- Age 34 years old
- Birthday April 29, 1979
A Spaniard living in Germany
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My research interests
Protein post translational modifications (phosphorylation, sumoylation, ubiquitination, acetylation).
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