Find content
Not Telling
leelee, on 09 August 2012 - 07:18 PM, said:
You could use a P200 and suck the clumps away individually 
Posts I've Made
In Topic: [Question] how to make primary-cultured cells evenly distributed?
11 August 2012 - 07:28 AM
Thanks for your attached file, bob1. Since this unevenly distribution is only seen in cardiomyocyte culture but not in my other cell cultures in the same incubator, I feel it may not be due to the mechanic vibration. I attached a sketch of the uneven cell seeding I encountered, trying to show the two problems when using a 4-chamber slide, tangled cells in patches (or maybe not digested completely? How do I know the digestion is just enough?) most observed in the middle of the chamber, and cells landed heavily around the well ( Was the way I shake the chamber slide wrong? It seems hard to handle containers with small wells, such as 24 well plates.)
Leelee, I use an intelli-mixer to rotate the tissuses at the speed of 4 RPM. After filtered the cells, however, I didn’t do so many times of pipetting as you said. I’ll pay attention to this next time and see if it helps, thanks.
And thanks you all, dear forum fellows.
Leelee, I use an intelli-mixer to rotate the tissuses at the speed of 4 RPM. After filtered the cells, however, I didn’t do so many times of pipetting as you said. I’ll pay attention to this next time and see if it helps, thanks.
And thanks you all, dear forum fellows.
