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"The pH may rise 0.1 to 0.3 units upon filtration" (This is found in many Tissue Culture publications.)
Example:
pH is adjusted to 7.00. After filter sterilization, the pH becomes 7.10-7.30.
My questions:
Why does the pH rise upon filtration? What's the mechanism behind it?
Does the pH rise only in medium containing sodium bicarbonate? Does it also rise in PBS (Na-Bicarb free)?
Any thoughts are welcome.
