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newbie99

Member Since 01 Sep 2009
Offline Last Active May 07 2012 05:52 PM
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Posts I've Made

In Topic: Making Phosphate buffer with calcium chloride

07 May 2012 - 05:55 PM

I make PBS+/+ all the time and I never see precipitates.

In Topic: Making Phosphate buffer with calcium chloride

26 April 2012 - 03:59 PM

The order is important. You add in three ingredients all at once, you'll get precipitation.

In Topic: Making Phosphate buffer with calcium chloride

25 April 2012 - 10:14 AM

Instead of using CaCl2 (FW 110.98), try CaCl2+2H2O (FW 147.01), you'll see the difference.

In Topic: Getting rid of percipitate in Ringer solution?

14 February 2012 - 07:04 AM

Could it be precipitation of calcium carbonate?

Add your chemicals in this order:
1- NaCl
2- KCl
Mix those two first. After they're completely dissolved, add
3- CaCl2-H2O
After it dissolves completely, finally add
4- NaHCO3

In Topic: How to prepare 1M EDTA with only Tris Base?

13 February 2012 - 10:57 AM

Thanks. I just realized that the authors of the Nature article probably didn't prepare the EDTA solution at 1M, rather at a more realistic molarity, because all EDTA mentioned in the paper are in diluted form.
It makes me wonder why they would include the recipe for a 1L 1M EDTA in the "Materials" section.

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