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Hi!
I wanted to do some "citizen science" combining a pretty well equiped lab and some pretty motivated high school students.
But what to do?
...we have reagents but little apparatus for analytical chemistry?
Is there a way to detect caffeine withouth a HPLC? Paper chromatography?
Can we measure water quality?
Food contents?
What would you do?
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Chemical analysis for a high school lab?
14 December 2012 - 07:47 AM
Python function to read multiple FASTA files and merging them into one dictionar
17 November 2012 - 03:44 AM
Hi guys, I'm just throwing this function up here to see if there are any Python aces out there,
Any ideas why this collapses???
cheers!
import sys, pdb
def FASTAs_to_dict(files):
+"""Appends FASTA files given as arguments to a dictionary"""
+ID = ""
+SEQ = ""
+for seqfile in files: #goes through each sequence in Sequences
++sequences = open(seqfile, 'r')
++for line in sequences:
+++if line.startswith(">"): #> saves previous entry, set new ID, clears SEQ
++++if ID != "": #only makes entries for ID with values
+++++FASTA_dict[ID] = SEQ
++++ID = line
++++SEQ = ""
+++else:
++++if line != "": #neccesary? ..does adding a "" string affect anything?
+++++SEQ += line
+return FASTA_dict
#main
FASTA_dict = {}
files=sys.argv[1:]
FASTAs_to_dict(files)
files.close
FASTA_dict = FASTAs_to_dict(files)
print FASTA_dict
Any ideas why this collapses???
cheers!
import sys, pdb
def FASTAs_to_dict(files):
+"""Appends FASTA files given as arguments to a dictionary"""
+ID = ""
+SEQ = ""
+for seqfile in files: #goes through each sequence in Sequences
++sequences = open(seqfile, 'r')
++for line in sequences:
+++if line.startswith(">"): #> saves previous entry, set new ID, clears SEQ
++++if ID != "": #only makes entries for ID with values
+++++FASTA_dict[ID] = SEQ
++++ID = line
++++SEQ = ""
+++else:
++++if line != "": #neccesary? ..does adding a "" string affect anything?
+++++SEQ += line
+return FASTA_dict
#main
FASTA_dict = {}
files=sys.argv[1:]
FASTAs_to_dict(files)
files.close
FASTA_dict = FASTAs_to_dict(files)
print FASTA_dict
Home-made Anti-bleach (?), vectashield and Immunocytochemistry
08 November 2012 - 04:30 AM
Hi all,
I have some ideas regarding immunocytochemistry.
They are more like questions actually.
Is there a good way to protect samples from bleaching? Samples with a bound flourescent secondary antibody?
I used to use vectashield in another lab but here everyone is using glycerol/PBS. Will there be a diffrence?
Can I add something to make it "vectashield-ish"?
From what I understtod vectashield are cheap chemicals sold expensively...
Cheers!
PS. And do I need to bother at all?
I have some ideas regarding immunocytochemistry.
They are more like questions actually.
Is there a good way to protect samples from bleaching? Samples with a bound flourescent secondary antibody?
I used to use vectashield in another lab but here everyone is using glycerol/PBS. Will there be a diffrence?
Can I add something to make it "vectashield-ish"?
From what I understtod vectashield are cheap chemicals sold expensively...
Cheers!
PS. And do I need to bother at all?
Bromine jar growing strange crystals...
03 November 2012 - 03:19 AM
Hi all,
I dont get why this jar has started growing crystals on the outside.
Its Bromine 99% (purissim) inside.
Could it be a leaking gas that crystalises on the outside?
What to do with it?
The jar is about 5-7 years old.
cheers!
I dont get why this jar has started growing crystals on the outside.
Its Bromine 99% (purissim) inside.
Could it be a leaking gas that crystalises on the outside?
What to do with it?
The jar is about 5-7 years old.
cheers!
Downloading a complete trace archive for a species
26 October 2012 - 05:49 AM
Hi,
I´m interested in downloading the complete EST sequences stored in trace archives in "BLAST".
Do you know how to do that?
cheers,
I´m interested in downloading the complete EST sequences stored in trace archives in "BLAST".
Do you know how to do that?
cheers,
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