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BSM

Member Since 19 Aug 2009
Offline Last Active Apr 24 2012 08:20 AM
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Posts I've Made

In Topic: Endofree Maxiprep tube volume

24 August 2011 - 08:09 AM

View Postlittle mouse, on 24 August 2011 - 06:06 AM, said:

It's true that some tubes need to be filled at 80%, unless they will collapse in the centrifuge.

You need to find other tubes, or to dilute your samples.



So a 5ml ethanol wash step could be replaced with 20-30ml ethanol without problems?

In Topic: Endofree Maxiprep tube volume

23 August 2011 - 07:34 PM

No it says 80% for proper centrifugation.

Since I plan on performing transfections + mouse microinjections in the future there is also a centrifuge here that can spin 50ml sterile conical vials, but it max RPM is 5300 (avg. g = 4894 and max g=6871). I guess this may work even though the avg g is not quite 5000 which is what the protocol calls for.

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