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Adrian K

Member Since 06 Aug 2009
Offline Last Active May 01 2013 08:09 AM
*****

Posts I've Made

In Topic: SDS page of acid soluble collagen

01 May 2013 - 02:32 AM

I guess after you had neutralized your acetic acid, the collagen precipitated?
Maybe you try to use urea/thiourea buffer with CHAPS?

In Topic: Literature quote guessing game

01 May 2013 - 02:09 AM

I don't understand what the quote means ... lolx... my search engine didn't explain it to me Posted Image

In Topic: doing PCR using PCR product , help plz !!

06 February 2013 - 04:54 PM

1) Another possibility of the reaction failure is due to too much carryover salt.
2) I would also concern about the error rate in your amplicons. I don't think the quality will be good if you intend to do DNA sequencing later.

Just my 2 cents.

In Topic: happy birthday, phage434

06 February 2013 - 04:44 PM

Happy belated Birthday phage!!!
I hope can become your apprentice in the soon future.
Posted Image

In Topic: Is anti-His antibody detergent sensitive?

06 February 2013 - 04:43 PM

Do you have a chromatography system? you can use it with column to purify his-tagged protein using gradient / step-wise elution, and do another round of ion exchange to further purify your protein.

I can give you some of my contact of suppliers if you are still in Malaysia, just pm me.

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