I guess after you had neutralized your acetic acid, the collagen precipitated?
Maybe you try to use urea/thiourea buffer with CHAPS?
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Hi, thanks for viewing my page...however I got not much to tell at the moment and pls come back later, will you? Cheers.
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In Topic: SDS page of acid soluble collagen
01 May 2013 - 02:32 AM
In Topic: Literature quote guessing game
01 May 2013 - 02:09 AM
I don't understand what the quote means ... lolx... my search engine didn't explain it to me
In Topic: doing PCR using PCR product , help plz !!
06 February 2013 - 04:54 PM
1) Another possibility of the reaction failure is due to too much carryover salt.
2) I would also concern about the error rate in your amplicons. I don't think the quality will be good if you intend to do DNA sequencing later.
Just my 2 cents.
2) I would also concern about the error rate in your amplicons. I don't think the quality will be good if you intend to do DNA sequencing later.
Just my 2 cents.
In Topic: happy birthday, phage434
06 February 2013 - 04:44 PM
Happy belated Birthday phage!!!
I hope can become your apprentice in the soon future.
I hope can become your apprentice in the soon future.
In Topic: Is anti-His antibody detergent sensitive?
06 February 2013 - 04:43 PM
Do you have a chromatography system? you can use it with column to purify his-tagged protein using gradient / step-wise elution, and do another round of ion exchange to further purify your protein.
I can give you some of my contact of suppliers if you are still in Malaysia, just pm me.
I can give you some of my contact of suppliers if you are still in Malaysia, just pm me.
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