i am doing SSCP technique for my PCR product, i make many gels and the bands separated well into 2 single stranded bands instead of one BUT now from 2 weeks ago the PCR bands didnt separated at all.
i try to change the polyacrylamide to a new one, change TEMED and always used freshly prepared APS and TRIS buffer but no separation and at the end i found that the current slowed down to 5 and then ZERO milliamp. and found no current passed across the gel.
i change the power supply and the electrodes
i think there is some wrong in the polyacrylamide gel it self but i don't know whats the problem
i will be highly thankful if anyone can help me or give me advice to do
waiting ur cooperation and reply
N.HussienMember Since 04 Aug 2009
Offline Last Active Jan 12 2013 12:14 PM
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05 Aug 2009 - 10:39