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Sudden changes also induce stress on the cells as they have to get used to different levels of certain compounds in the medium. This means that some of the cells will die, and you will be left with a population of cells that are resistant to those changes - you just changed the cell line, so that it is no longer what you thought it was...
To change cells into different media, you need to do this slowly over several passages using increasing amounts of the new medium and decreasing amounts of the old medium. This is referred to as "weaning" the cells into a new medium. After this process, you should characterize the cells for morphology, growth rate, expression of various genes to ensure that the cells have not changed significantly from the parent line. This is also important to ensure that your experiments are not going to be affected by the changes!
#139803 What will happen if the cell culture medium is suddenly changed to another one?
Posted
on 22 August 2012 - 12:37 PM
