Sudden changes also induce stress on the cells as they have to get used to different levels of certain compounds in the medium. This means that some of the cells will die, and you will be left with a population of cells that are resistant to those changes - you just changed the cell line, so that it is no longer what you thought it was...
To change cells into different media, you need to do this slowly over several passages using increasing amounts of the new medium and decreasing amounts of the old medium. This is referred to as "weaning" the cells into a new medium. After this process, you should characterize the cells for morphology, growth rate, expression of various genes to ensure that the cells have not changed significantly from the parent line. This is also important to ensure that your experiments are not going to be affected by the changes!
asliMember Since 30 Jul 2009
Offline Last Active Nov 28 2012 03:11 AM
- Group Active Members
- Active Posts 17
- Profile Views 2,756
- Member Title member
- Age Age Unknown
- Birthday Birthday Unknown
My research interests
Liposome, colorectal cancer cell lines