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This is why I'm wondering. If the transfection didn't work, I shouldn't be seeing an increase in expression.
But my GOI is suppose to increase proliferation. Instead of cell growing, I see cells floating.
Weird...
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In Topic: Tet-On inducible system
10 March 2013 - 07:23 PM
Yupe. I transfected my cell lines with transactivator vector. The expression increased with higher doxycycline induction. But at the same time I see a lot of dead cells floating.
This is why I'm wondering. If the transfection didn't work, I shouldn't be seeing an increase in expression.
But my GOI is suppose to increase proliferation. Instead of cell growing, I see cells floating.
Weird...
This is why I'm wondering. If the transfection didn't work, I shouldn't be seeing an increase in expression.
But my GOI is suppose to increase proliferation. Instead of cell growing, I see cells floating.
Weird...
