Yupe. I transfected my cell lines with transactivator vector. The expression increased with higher doxycycline induction. But at the same time I see a lot of dead cells floating.
This is why I'm wondering. If the transfection didn't work, I shouldn't be seeing an increase in expression.
But my GOI is suppose to increase proliferation. Instead of cell growing, I see cells floating.
sanjiunMember Since 16 Jul 2009
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My research interests
Genomics, gene expression, immunology, host-pathogen interaction