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megashock

Member Since 12 Jul 2009
Offline Last Active May 02 2012 10:57 AM
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Posts I've Made

In Topic: Counting and plating cells

28 February 2012 - 10:27 PM

Thanks

I will take the cells volume that i need in PBS according to the cell count and than centrifuge, then take out the PBS and add DMEM+FBS.
The concentration maybe will change, but the cell count will stay the same.

I have another question.
Does leaving the cells for a long time in PBS (1-2 hours) will harm them ?
because that i have a lot of cells to count and it may take a while before I count all the plates.

In Topic: Calculating the amount of the desired cells in the medium ?

28 February 2012 - 12:09 AM

Thanks that is exactly what i did.
there's an on-line calculator that calculates this.

http://www.physiolog...per_volume.html

Another similar calculation that i did That can be in interest, was to count the cells and than to take the desired number of cells without dillution.
It's basically the same calculation.

If i have 1000 cells in 1ml and i need to take 50 cells.
C1V1=C2V2
x=50*1/1000 => take 0.05ml (50 micL) and you will have 50 cells.

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