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Vassil

Member Since 09 Jul 2009
Offline Last Active May 03 2013 10:22 AM

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#143749 miRNA qPCR normalization/control gene sequence!!!!

Posted Neanderthal on 19 October 2012 - 10:18 AM

Please see the link below:

http://www3.appliedb.../cms_044972.pdf

A list of controls are mentioned in this PDF. Hope it helps.

Thanks,

K.

#141918 siRNA modifications

Posted pcrman on 20 September 2012 - 12:13 PM

For general in vitro use, you don't need chemical modification of siRNA because it is quite stable over a few years if properly stored.

If you really want to introduce modification, 2'OMe or 2'F modification should be good. If the mod is on the passenger strand, it can also reduce offtarget effects.

#134258 PCR product purification

Posted memari on 09 May 2012 - 08:49 AM

We have used this and now we have 5 fold more DNA for QPCR for Chip. It is definitly better tha QIAGEN.
Feldan's PCR Purification Kit
http://www.feldan.co...on-kit-200preps

Babak